aml-12 mouse hepatocyte cell line Search Results


mouse hepatocyte cell line aml12  (Lonza)
90
Lonza mouse hepatocyte cell line aml12
A: Nitrite production after 24-h incubation of RAW 264.7 cells with <t>AML12</t> hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.
Mouse Hepatocyte Cell Line Aml12, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse hepatocyte cell line aml12 - by Bioz Stars, 2026-02
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A: Nitrite production after 24-h incubation of RAW 264.7 cells with AML12 hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.

Journal: Journal of Clinical and Translational Research

Article Title: IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man

doi:

Figure Lengend Snippet: A: Nitrite production after 24-h incubation of RAW 264.7 cells with AML12 hepatocyte-derived DAMPs. Data are plotted as mean ± SEM with N = 7-8 per group. B: Real-time ROS production after 24-h incubation of RAW 264.7 cells with AML12-derived DAMPs. Data are plotted as mean ± SEM with N = 4 per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001 compared to the medium group. Abbreviations: c, centrifuged; LPS, lipopolysaccharide; PMA, phorbol 12-myristate 13-acetate; DCF, dichlorofluorescein.

Article Snippet: The mouse hepatocyte cell line AML12 was cultured in William’s E (WE) medium (Lonza, Basel, Switzerland) supplemented with 10% (v/v) fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA), penicillin (100 U/mL, Thermo Fisher Scientific), streptomycin (100 μg/mL, Thermo Fisher Scientific), insulin (5 μg/mL, Sigma-Aldrich, St. Louis, MO), L-glutamine (2 mM, Thermo Fisher Scientific), and hydrocortisone hemisuccinate (50 μM, Sigma-Aldrich).

Techniques: Incubation, Derivative Assay

Pro-inflammatory signaling by DAMP-exposed RAW 264.7 macrophages. A: TNFα, B: IL-1β, and C: IL-6 mRNA expression after 1-h and 6-h DAMP incubation. All results are presented as fold upregulation compared to medium incubation (N = 3-4 per group). D-G: Luciferase reporter assay of RAW 264.7 NF-κB/LUCPorter cells following medium-, DAMP-, or LPS stimulation after D: 6 h, E: 12 h, and F: 24 h (N = 3 per group). G: Luciferase reporter assay after stimulation with DAMPs derived from ischemia-subjected necrotic cells, medium, or LPS after 24 h of exposure (N = 3 per group). Luciferase activity is expressed as the fold change relative to control. H: IL-23 production by murine macrophages in response to AML12 hepatocyte-derived DAMPs measured by ELISA in RAW 264.7 cell supernatant and corrected for protein (N = 4 per group). All data represent mean ± SEM. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 compared to the medium samples.

Journal: Journal of Clinical and Translational Research

Article Title: IL-23 and IL-17A are not involved in hepatic/ischemia reperfusion injury in mouse and man

doi:

Figure Lengend Snippet: Pro-inflammatory signaling by DAMP-exposed RAW 264.7 macrophages. A: TNFα, B: IL-1β, and C: IL-6 mRNA expression after 1-h and 6-h DAMP incubation. All results are presented as fold upregulation compared to medium incubation (N = 3-4 per group). D-G: Luciferase reporter assay of RAW 264.7 NF-κB/LUCPorter cells following medium-, DAMP-, or LPS stimulation after D: 6 h, E: 12 h, and F: 24 h (N = 3 per group). G: Luciferase reporter assay after stimulation with DAMPs derived from ischemia-subjected necrotic cells, medium, or LPS after 24 h of exposure (N = 3 per group). Luciferase activity is expressed as the fold change relative to control. H: IL-23 production by murine macrophages in response to AML12 hepatocyte-derived DAMPs measured by ELISA in RAW 264.7 cell supernatant and corrected for protein (N = 4 per group). All data represent mean ± SEM. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 compared to the medium samples.

Article Snippet: The mouse hepatocyte cell line AML12 was cultured in William’s E (WE) medium (Lonza, Basel, Switzerland) supplemented with 10% (v/v) fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA), penicillin (100 U/mL, Thermo Fisher Scientific), streptomycin (100 μg/mL, Thermo Fisher Scientific), insulin (5 μg/mL, Sigma-Aldrich, St. Louis, MO), L-glutamine (2 mM, Thermo Fisher Scientific), and hydrocortisone hemisuccinate (50 μM, Sigma-Aldrich).

Techniques: Expressing, Incubation, Luciferase, Reporter Assay, Derivative Assay, Activity Assay, Control, Enzyme-linked Immunosorbent Assay